MbShea@PWP@English@UMCP

Working Title: Site-Directed Mutagenesis of Lysine 104 and 157 do not inhibit the function of Chlamydia Diaminopimelate epimerase

Audience: Researchers in the field of microbiology, specifically studying Chlamydia trachomatis

Context: Chlamydia trachomatis is the most prevalent sexually transmitted infection in the United States. According to the Centers for Disease Control and Prevention, there were over 1.7 million reported cases of chlamydial infections in the 50 states and the District of Columbia in 2017. This estimate is lower than the real total number of cases of chlamydial infections as many people are often unaware that they contracted the infection.

Purpose: The purpose of this research paper is to report the findings of my research from this past summer at the Uniformed Services University of the Health Sciences. Through this research experience, I was responsible for mutating a specific bifunctional gene in Chlamydia trachomatis to determine if site-directed mutagenesis would result in lower cell counts.

DOCUMENT TYPE: Research Paper

Design/format: Two-column format with images embedded

Citation style: APA Format

Seems like this was a really interesting project! I would be interested to learn more about the significance of these particular mutations. Did you target them because they are clinically relevant?

J may have given you an opening or closing to your document. Can you address that? With some stats or problem description about the clinical need?

Good plan, N; glad you write for a read audience.