FINAL PROJECT PROPOSAL: Magnus Opus and Exigence > Laboratory Manual for Validating the FLT3 F691L Mutation in Acute Myeloid Leukemia
C, this is your lab, right? Can you be specific or do you have privacy concerns (see me right away!).
Are you proposing to fill this gap of procedural documentation entirely OR are you supplementing what is already written?
You can also help students see the status of personalized genomics and particular cancers. For example, this is a helpful review. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352312/
And, a way to close this would be what is coming next: next gen sequencing, insurance coverage, linking with immunotherapy, etc.
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December 1, 2019 |
Marybeth Shea
Marybeth Shea
Audience:
This paper will be written for beginner researchers or interns working in labs striving towards identifying specific mutations in patients with different kinds of cancer. The audience is assumed to have basic biology laboratory skills and knowledge and at least a small foundation of cancer research.
Context:
Cancer research has become a main focus in today’s science/research world due to its large impacts on the society as a whole as well as most people individually. Patients with cancer can have mutations even as small as a point mutation that can cause them to be resistant to treatments. Being able to test for and detect these mutations early on can allow for a form of “personalized” medicine, in which patients can receive proper treatment (drugs they aren’t resistant towards). Therefore, this laboratory manual will act as an example for developing assays that can be used to evaluate patient samples for certain mutations that can confer resistance to specific cancer drugs.
Purpose:
The purpose of this laboratory manual is to give beginner researchers insight on how to develop an assay to detect specific mutations. The hope is that this laboratory manual can be used as a basis so that other beginner researchers can modify this procedure to work with other mutations associated with AML and possibly other cancers.
Document Type:
A Laboratory Manual
Design/Format:
This laboratory manual will provide a short section of background information about acute myeloid leukemia, the different kinds of mutations associated with the FLT3 protein in patients with AML, and different kinds of inhibitors. The next section will consist of two or three procedures used to validate and sequence the FLT3 F691L mutation. The third section will include techniques for evaluating the clinical significance of mutations and databases that can be used to gather this information.
Citation Style:
APA citations will be used for the first section (background). Some referral links will be used in the second section (procedure). A combination of APA citations along with some referral links to databases will be used in the last section (clinical significance).