MbShea@PWP@English@UMCP

Abstract
The RIO Kinases are a family of protein kinases that are heavily involved (in eukaryotes) in the generation and maintenance of ribosomes in cells. Dr. LaRonde (your future PI) has been conducting research into the molecular structure and function of the RIO Kinases for the larger part of the last 15 years. In her research, Dr. LaRonde found a deep molecular homology of the RIO Kinases, and she emphasized that this homology could be crucial in determining the molecular structure of the three major RIO Kinases (RIO1, RIO2, RIO3). All three of these protein kinases have been found to be common causes of the metastasis of pancreatic, lung, and breast cancers. As a result, Dr. LaRonde’s lab is investigating the molecular structure of these protein kinases, as well as the structure of the protein kinases in complex with anticancer research drugs (from the NIH). Dr. LaRonde has already successfully identified the structure of the RIO1 Kinase, as well as an anticancer drug, toyocamycin, that inhibits the function of the protein kinase. In Dr. LaRonde’s lab, you will likely be assisting in the crystal analysis of the RIO2 and RIO3 protein kinases, as well as in the identification of research drugs that inhibit the function of these kinases.
Reader’s Profile: I imagine a reader skeptical of the content of the research (mainly Dr. LaRonde), who would emphasize that I include more information on the Biochemistry side of the research.
Reader’s Respone: I really like how he included research on the prevalence of the overexpression of RIO Kinases in three specific types of cancer (lung, breast, and pancreatic). I think that in the future, he should add more information on the molecular homology of the RIO Kinase family, as our lab is also seeking to learn more about the function of RIOK3 in eukaryotic organisms. Alex’s literature review seems to be somewhat lacking on the research into the molecular structure of the RIO kinases, but I expect that Nishat will help him out over the summer with this analysis. As he learns to analyze crystal (protein) structures, I expect that he will get a better understanding of the molecular mechanisms which the RIO Kinases use to function normally.
Revised thesis or problem statement: My thesis/problem statement hasn’t really changed much, however, I have decided to narrow down the cancers that I am looking at to lung, breast, and pancreatic cancers, as these are the cancers that 2 or more of the RIO Kinases are involved in.
Voice: My voice will be semi-formal throughout, and it will become more formal at parts that are very focused on analysis of the literature on RIO kinases.
Citation: The citations will mostly be done naturally in my sentence writing (“Dr. LaRonde cites that…”), though I will include a references page at the end of my document.

A, nicely described. Your reader might be helped with a cover and closing note from you. Can then place some of this in a time line of research and that the lab staff sometimes divide the topics for efficiency and for shared labor.

Looking forward to this guide.

And, so, too, would be Dr. L. And, unknown future student researchers.