Post Here: Definition Topic/Audiences/Exigence > Illumina Ultra High Throughput DNA Sequencing and Its Applications in early cancer┬ádetection

Title: Illumina Ultra High Throughput DNA Sequencing and Its Applications in early cancer detection

Audience: I am writing for professionals in the field that may not be familiar with the technology, especially those that work in labs that perform any kind of DNA sequencing. The audience that I am writing for will require previous knowledge about traditional sequencing methods (e.g. Sanger sequencing) and basic genetics. Secondary audience: Students learning about Illumina sequencing in class.

Context: DNA sequencing is a relatively recent technological innovation that has revolutionized many industries including ( but not exclusive to) human genetics and genomics, agriculture, virology, and environmental genomics. In the past decade DNA sequencing technology has improved at such a high rate that commercial use of DNA sequencing appears potentially feasible. With Illumina sequencing 4 to 48 samples can be run simultaneously, producing typing results for 11 loci. Unlike traditional sequencing methods, which could take days to weeks at least, these results can be reported in less than four days. As the technology advances, it is becoming increasingly faster, cheaper, and more accessible. Illumina sequencing, and Next Generation Sequencing (NGS) in general, will enable discoveries that can hopefully be translated into powerful cancer diagnostic tools.

Purpose: The purpose of this document is to teach the methods of Illumina sequencing, and discuss its potential diagnostic abilities. Information will be gathered from primary sources when possible, and from other scholarly sources to compile knowledge about the Illumina system. Another purpose is to discuss the possible concerns this technology may give rise to.


Document Type: Possibly a medical school seminar paper.
November 20, 2015 | Unregistered CommenterJL